A NEW COLORIMETRIC METHOD FOR THE DETERMINATION OF SERUM PROTEIN

New colorimetric method for quantitative determination of protein in urine.

Total urinary protein is rapidly precipitated at room temperature by tannic acid. The tannic acid/protein precipitate, dissolved in aqueous triethanolamine/ferric chloride solution, gives a purple-violet color of high absorptivity. Absorbance at 510 nm is linearly related to concentration from 0.05 to 1.50 A for a protein content of 0.05 to 1.50 g/liter, and less than 5 mg/liter can be detected...

New Enzymatic Colorimetric Method for the Quantitative Determination of Phenylalanine in Dry-Blood Spots

Phenylketonuria (PKU) is an autosomal recessive disorder, which is characterized by severe mental retardation, microcephaly and seizures. The symptoms of this disease can be prevented if detected soon after birth. Therefore, blood Phenylalanine (Phe) measurement is essential for the early diagnosis, treatment and dietary monitoring of PKU patients. The goal of this research was to introduce a r...

A Colorimetric Method for Determination of Serum Chloride.

HAVE INSTITUTED a colorimetric chloride procedure which has been in use for 3 years in our laboratory and has met with great success. This colorimetric method is based on a procedure used in automated syStems* (2, 3). It is extremely simple, quite accurate, and gives highly reproducible results even in the hands of student technicians. Those laboratories not having access to a chloridometer or ...

A simple method for the determination of serum cholesterol

A new simple semimicro method for colorimetric determination of urea.